Research Techniques

Small animal microsurgery

• Abdominal aortic aneurysm (elastase perfusion model, elastase topical application model, calcium chloride model, angiotensin II model, BAPN rupture model)
• Thoracic aortic aneurysm (elastase model only)
• Aortic transplant
• Heterotopical heart transplant (abdominal model, cervical model, re-transplant, second-graft transplant, target gene transfection)
• Other rodent survival surgeries (e.g. orchiectomy, ovariectomy, drug pellet or device implant etc.)

Large animal surgery

• Aortic aneurysm induction, organ or tissue transplants & other survival surgeries

Molecular Biology

• Protein, RNA and DNA abstraction
• Western blot
• RT-PCR
• Gelatin zymography & reverse zymography
• Cytokine and angiogenesis array
• ELISA
• Elispot
• Flow cytometry

Cell culture

• Isolation and culture of vascular smooth muscle cell
• Stem cell culture and application
• Bone marrow transplantation
• Adoptive T cell or B cell transfer

Histology

• Tissue sample processing and slice preparations
• Regular histology stainings (HE, Verhoeff van Gieson, Picrosirius red, Trichrome, etc.) 
• Immunohistochemical stainings (neutrophil, macrophage,CD3⁺ T cell, α-smooth muscle cell actin)
• Fluorescent & confocal imaging

Miscellaneous lab skills

• Small animal ultrasound
• Murine colony management and genotyping
• Sample collecting (tail vein, retro-orbital, lavage)
• Drug dosing (I.V., I.P., I.H, gavage)