Research Techniques

Immunohistochemistry of murine lungs showing increased neutrophil staining after IR compared to sham (controls).
Immunohistochemistry of murine lungs showing increased neutrophil staining after IR compared to sham (controls).
Isolation and characterization of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) by flow cytometry (A), Nanosight (B-C) and Imagestream analysis (D). EV content includes protein and RNA (E).
Isolation and characterization of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) by flow cytometry (A), Nanosight (B-C) and Imagestream analysis (D). EV content includes protein and RNA (E).

Cell & Molecular Techniques

Luminex cytokine arrays, exosome isolation, immunohistochemistry, immunofluorescence, flow cytometry, precision cut lung slices, RNA sequencing, real-time PCR, micro RNA assays, calcium assays, confocal microscopy

Murine Techniques

Transgenic/knockout murine studies, pulmonary function analysis, ex vivo lung perfusion, lung histology and confocal microscopy, bronchoalveolar lavage, adoptive transfer of immune cells, PET CT imaging