Ashish K Sharma

Ashish K Sharma, MD, PhD

Associate Professor

Department: MD-SURGERY-VASCULAR
Business Phone: (352) 294-8660

About Ashish K Sharma

Dr. Sharma is a basic and translational medical scientist with a background in immunology, cell biology, molecular biology and pulmonary physiology. He joined UF in June 2018 as an Associate Professor in the Department of Surgery. Prior to joining UF, Dr. Sharma was a faculty member in Department of Surgery at University of Virginia since 2004. He received his PhD from University of Virginia and completed his medical graduation from MR Medical College, Gulbarga, India.

The research in Dr. Sharma’s laboratory focuses on defining the molecular and signal transduction mechanisms of acute lung injury (i.e. ischemia-reperfusion injury or primary graft dysfunction) after lung transplantation as well as investigating the pathophysiology of aortic aneurysms. The main focus of his research is to decipher the contribution of purinergic signaling via pannexin and transient receptor potential channels as well as to implement mesenchymal stem cell-derived extracellular vesicles as a therapeutic strategy in aortic aneurysms and primary graft dysfunction after lung transplantation.

He has served as a peer-reviewer for numerous scientific journals. He is an active member of the American Thoracic Society and the American Heart Association and has been invited to give guest lectures at various academic institutions and to serve as a guest editor on peer-reviewed scientific journals. Dr. Sharma has a strong history in the research training of surgery residents, postdoctoral fellows, graduate students, medical students and undergraduates.

Accomplishments

Alan T. Hirsch, MD Award in Vascular Medicine
2019 · American Heart Association
RO1 Research Grant (Principal Investigator)
2018 · National Institute of Health (NIH/NHLBI)
Dean's Excellence in Team Science Research Award
2017 · University of Virginia
Grant-in-Aid
2017 · American Heart Association
Conrad Jobst Award for outstanding contributions in vascular research
2016 · Coller Surgical Society, University of Michigan
Top 10 Abstract Award
2015 · American Heart Association
Peripheral Vascular Disease Council Award
2014 · Arteriosclerosis, Thrombosis, and Vascular Biology

Research Profile

Dr. Sharma’s research laboratory focuses on the role of pannexin and transient receptor channel signaling in aortic aneurysms and lung transplant immunology. Furthermore, he is also investigating the role of mesenchymal stem cells and its derivatives i.e. extracellular vesicles, as a therapeutic modality in the treatment of lung injury and aortic aneurysms. In addition, Dr. Sharma has extensive expertise in the role of innate immune cells, cytokines, HMGB1/RAGE axis, purinergic signaling and oxidative stress during inflammation and vascular remodeling in acute lung injury as well as aortic aneurysms. He has extensively published manuscripts, book chapters and abstracts on these topics.

Another area of research is the rehabilitation of non-heart beating donor lungs (i.e. DCD or donation after cardiac death) via ex vivo lung perfusion (EVLP) which has the potential to increase the donor lung pool size. Dr. Sharma’s research focuses on the use of stem cells and extracellular vesicles to supplement EVLP as a strategy to rescue marginal donor lungs and to prevent primary graft dysfunction. These projects utilize mouse and pig models of lung transplant & ischemia-reperfusion injury, mouse/pig/human EVLP models and in vitro models.

Publications

2021
A novel injury site-natural antibody targeted complement inhibitor protects against lung transplant injury.
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 21(6):2067-2078 [DOI] 10.1111/ajt.16404. [PMID] 33210808.
2021
In vivo reprogramming of pathogenic lung TNFR2+ cDC2s by IFNβ inhibits HDM-induced asthma.
Science immunology. 6(61) [DOI] 10.1126/sciimmunol.abi8472. [PMID] 34244314.
2021
Maresin 1 activates LGR6 signaling to inhibit smooth muscle cell activation and attenuate murine abdominal aortic aneurysm formation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 35(8) [DOI] 10.1096/fj.202100484R. [PMID] 34320253.
2021
Mesenchymal Stem Cells Alter MicroRNA Expression and Attenuate Thoracic Aortic Aneurysm Formation.
The Journal of surgical research. 268:221-231 [DOI] 10.1016/j.jss.2021.06.057. [PMID] 34371281.
2020
Female Mice Exhibit Abdominal Aortic Aneurysm Protection in an Established Rupture Model.
The Journal of surgical research. 247:387-396 [DOI] 10.1016/j.jss.2019.10.004. [PMID] 31699539.
2020
MicroRNA-206 antagomiR‒enriched extracellular vesicles attenuate lung ischemia‒reperfusion injury through CXCL1 regulation in alveolar epithelial cells.
The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation. 39(12):1476-1490 [DOI] 10.1016/j.healun.2020.09.012. [PMID] 33067103.
2020
Pharmacologic inhibition of transient receptor channel vanilloid 4 attenuates abdominal aortic aneurysm formation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 34(7):9787-9801 [DOI] 10.1096/fj.202000251R. [PMID] 32506673.
2020
Single-Photon Emission Computed Tomography Imaging Using Formyl Peptide Receptor 1 Ligand Can Diagnose Aortic Aneurysms in a Mouse Model.
The Journal of surgical research. 251:239-247 [DOI] 10.1016/j.jss.2020.01.028. [PMID] 32172010.
2019
A novel swine model of abdominal aortic aneurysm.
Journal of vascular surgery. 70(1):252-260.e2 [DOI] 10.1016/j.jvs.2018.09.057. [PMID] 30591288.
2019
Adenosine 2A Receptor Activation Attenuates Ischemia Reperfusion Injury During Extracorporeal Cardiopulmonary Resuscitation.
Annals of surgery. 269(6):1176-1183 [DOI] 10.1097/SLA.0000000000002685. [PMID] 31082918.
2019
Cyclophilin A contributes to aortopathy induced by postnatal loss of smooth muscle TGFBR1.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 33(10):11396-11410 [DOI] 10.1096/fj.201900601RR. [PMID] 31311317.
2019
ZFP148 (Zinc-Finger Protein 148) Binds Cooperatively With NF-1 (Neurofibromin 1) to Inhibit Smooth Muscle Marker Gene Expression During Abdominal Aortic Aneurysm Formation.
Arteriosclerosis, thrombosis, and vascular biology. 39(1):73-88 [DOI] 10.1161/ATVBAHA.118.311136. [PMID] 30580567.
2018
Ex Vivo Assessment of Porcine Donation After Circulatory Death Lungs That Undergo Increasing Warm Ischemia Times.
Transplantation direct. 4(12) [DOI] 10.1097/TXD.0000000000000845. [PMID] 30584586.
2018
Expanding the donor lung pool: how many donations after circulatory death organs are we missing?
The Journal of surgical research. 223:58-63 [DOI] 10.1016/j.jss.2017.09.029. [PMID] 29433886.
2018
Human mesenchymal stromal cell-derived extracellular vesicles attenuate aortic aneurysm formation and macrophage activation via microRNA-147.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. [DOI] 10.1096/fj.201701138RR. [PMID] 29812968.
2018
Increasing circulating sphingosine-1-phosphate attenuates lung injury during ex vivo lung perfusion.
The Journal of thoracic and cardiovascular surgery. 156(2):910-917 [DOI] 10.1016/j.jtcvs.2018.02.090. [PMID] 29609890.
2018
Invited commentary.
Journal of vascular surgery. 67(3) [DOI] 10.1016/j.jvs.2016.12.064. [PMID] 29477203.
2018
In vivo lung perfusion rehabilitates sepsis-induced lung injury.
The Journal of thoracic and cardiovascular surgery. 155(1):440-448.e2 [DOI] 10.1016/j.jtcvs.2017.08.124. [PMID] 29033043.
2018
Pannexin-1 channels on endothelial cells mediate vascular inflammation during lung ischemia-reperfusion injury.
American journal of physiology. Lung cellular and molecular physiology. 315(2):L301-L312 [DOI] 10.1152/ajplung.00004.2018. [PMID] 29745255.
2018
Protecting Donor Livers During Normothermic Machine Perfusion With Stem Cell Extracellular Vesicles.
Transplantation. 102(5):725-726 [DOI] 10.1097/TP.0000000000002124. [PMID] 29406445.
2018
Resolvin D1 decreases abdominal aortic aneurysm formation by inhibiting NETosis in a mouse model.
Journal of vascular surgery. 68(6S):93S-103S [DOI] 10.1016/j.jvs.2018.05.253. [PMID] 30470363.
2018
Tamsulosin attenuates abdominal aortic aneurysm growth.
Surgery. 164(5):1087-1092 [DOI] 10.1016/j.surg.2018.06.036. [PMID] 30174141.
2017
Airway pressure release ventilation during ex vivo lung perfusion attenuates injury.
The Journal of thoracic and cardiovascular surgery. 153(1):197-204 [DOI] 10.1016/j.jtcvs.2016.09.029. [PMID] 27742245.
2017
Ex Vivo Lung Perfusion Rehabilitates Sepsis-Induced Lung Injury.
The Annals of thoracic surgery. 103(6):1723-1729 [DOI] 10.1016/j.athoracsur.2017.01.018. [PMID] 28434548.
2017
Influenza A induces dysfunctional immunity and death in MeCP2-overexpressing mice.
JCI insight. 2(2) [DOI] 10.1172/jci.insight.88257. [PMID] 28138553.
2017
Lungs donated after circulatory death and prolonged warm ischemia are transplanted successfully after enhanced ex vivo lung perfusion using adenosine A2B receptor antagonism.
The Journal of thoracic and cardiovascular surgery. 154(5):1811-1820 [DOI] 10.1016/j.jtcvs.2017.02.072. [PMID] 28483262.
2017
Mesenchymal stromal cell-derived extracellular vesicles attenuate lung ischemia-reperfusion injury and enhance reconditioning of donor lungs after circulatory death.
Respiratory research. 18(1) [DOI] 10.1186/s12931-017-0704-9. [PMID] 29268735.
2017
Muscle-derived extracellular superoxide dismutase inhibits endothelial activation and protects against multiple organ dysfunction syndrome in mice.
Free radical biology & medicine. 113:212-223 [DOI] 10.1016/j.freeradbiomed.2017.09.029. [PMID] 28982599.
2016
Attenuation of Pulmonary Ischemia-Reperfusion Injury by Adenosine A2B Receptor Antagonism.
The Annals of thoracic surgery. 102(2):385-393 [DOI] 10.1016/j.athoracsur.2016.02.060. [PMID] 27109193.
2016
Ex vivo lung perfusion with adenosine A2A receptor agonist allows prolonged cold preservation of lungs donated after cardiac death.
The Journal of thoracic and cardiovascular surgery. 151(2):538-45 [DOI] 10.1016/j.jtcvs.2015.07.075. [PMID] 26323621.
2016
Mechanisms of lung ischemia-reperfusion injury.
Current opinion in organ transplantation. 21(3):246-52 [DOI] 10.1097/MOT.0000000000000304. [PMID] 26945320.
2016
Mesenchymal Stem Cells Attenuate NADPH Oxidase-Dependent High Mobility Group Box 1 Production and Inhibit Abdominal Aortic Aneurysms.
Arteriosclerosis, thrombosis, and vascular biology. 36(5):908-18 [DOI] 10.1161/ATVBAHA.116.307373. [PMID] 26988591.
2016
NOX2 Activation of Natural Killer T Cells Is Blocked by the Adenosine A2A Receptor to Inhibit Lung Ischemia-Reperfusion Injury.
American journal of respiratory and critical care medicine. 193(9):988-99 [DOI] 10.1164/rccm.201506-1253OC. [PMID] 26757359.
2015
Attenuation of aortic aneurysms with stem cells from different genders.
The Journal of surgical research. 199(1):249-58 [DOI] 10.1016/j.jss.2015.04.025. [PMID] 25958166.
2015
Donor Hepatic Steatosis Induce Exacerbated Ischemia-Reperfusion Injury Through Activation of Innate Immune Response Molecular Pathways.
Transplantation. 99(12):2523-33 [DOI] 10.1097/TP.0000000000000857. [PMID] 26285018.
2015
Ex Vivo Perfusion With Adenosine A2A Receptor Agonist Enhances Rehabilitation of Murine Donor Lungs After Circulatory Death.
Transplantation. 99(12):2494-503 [DOI] 10.1097/TP.0000000000000830. [PMID] 26262504.
2015
Increased 18F-FDG uptake is predictive of rupture in a novel rat abdominal aortic aneurysm rupture model.
Annals of surgery. 261(2):395-404 [DOI] 10.1097/SLA.0000000000000602. [PMID] 24651130.
2015
Sphingosine-1-phosphate receptor 1 agonism attenuates lung ischemia-reperfusion injury.
American journal of physiology. Lung cellular and molecular physiology. 308(12):L1245-52 [DOI] 10.1152/ajplung.00302.2014. [PMID] 25910934.
2014
NADPH oxidase mediates synergistic effects of IL-17 and TNF-α on CXCL1 expression by epithelial cells after lung ischemia-reperfusion.
American journal of physiology. Lung cellular and molecular physiology. 306(1):L69-79 [DOI] 10.1152/ajplung.00205.2013. [PMID] 24186876.
2013
Adenosine 2A receptor modulates inflammation and phenotype in experimental abdominal aortic aneurysms.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 27(6):2122-31 [DOI] 10.1096/fj.12-214197. [PMID] 23413358.
2013
Adenosine A1 receptor activation attenuates lung ischemia-reperfusion injury.
The Journal of thoracic and cardiovascular surgery. 145(6):1654-9 [DOI] 10.1016/j.jtcvs.2013.01.006. [PMID] 23398646.
2013
Adenosine A3 receptor activation attenuates lung ischemia-reperfusion injury.
The Annals of thoracic surgery. 95(5):1762-7 [DOI] 10.1016/j.athoracsur.2013.01.059. [PMID] 23541429.
2013
Apoptotic cell clearance by bronchial epithelial cells critically influences airway inflammation.
Nature. 493(7433):547-51 [DOI] 10.1038/nature11714. [PMID] 23235830.
2012
Acute hyperglycemic exacerbation of lung ischemia-reperfusion injury is mediated by receptor for advanced glycation end-products signaling.
American journal of respiratory cell and molecular biology. 46(3):299-305 [DOI] 10.1165/rcmb.2011-0247OC. [PMID] 21980055.
2012
Ex vivo rehabilitation of non-heart-beating donor lungs in preclinical porcine model: delayed perfusion results in superior lung function.
The Journal of thoracic and cardiovascular surgery. 144(5):1208-15 [DOI] 10.1016/j.jtcvs.2012.07.056. [PMID] 22944084.
2012
Experimental abdominal aortic aneurysm formation is mediated by IL-17 and attenuated by mesenchymal stem cell treatment.
Circulation. 126(11 Suppl 1):S38-45 [PMID] 22965992.
View on: PubMed
2011
Autocrine regulation of pulmonary inflammation by effector T-cell derived IL-10 during infection with respiratory syncytial virus.
PLoS pathogens. 7(8) [DOI] 10.1371/journal.ppat.1002173. [PMID] 21829368.
2011
Depletion of tissue plasminogen activator attenuates lung ischemia-reperfusion injury via inhibition of neutrophil extravasation.
American journal of physiology. Lung cellular and molecular physiology. 300(5):L718-29 [DOI] 10.1152/ajplung.00227.2010. [PMID] 21378024.
2011
Natural killer T cell-derived IL-17 mediates lung ischemia-reperfusion injury.
American journal of respiratory and critical care medicine. 183(11):1539-49 [DOI] 10.1164/rccm.201007-1173OC. [PMID] 21317314.
2011
Pretreatment strategy with adenosine A2A receptor agonist attenuates reperfusion injury in a preclinical porcine lung transplantation model.
The Journal of thoracic and cardiovascular surgery. 142(4):887-94 [DOI] 10.1016/j.jtcvs.2011.06.015. [PMID] 21762933.
2011
Suppression of signal transducer and activator of transcription 3 activation by butein inhibits growth of human hepatocellular carcinoma in vivo.
Clinical cancer research : an official journal of the American Association for Cancer Research. 17(6):1425-39 [DOI] 10.1158/1078-0432.CCR-10-1123. [PMID] 21131551.
2011
Ursolic acid inhibits multiple cell survival pathways leading to suppression of growth of prostate cancer xenograft in nude mice.
Journal of molecular medicine (Berlin, Germany). 89(7):713-27 [DOI] 10.1007/s00109-011-0746-2. [PMID] 21465181.
2011
Use of autogenous internal iliac artery for bridging the external iliac artery after excision of Aspergillus mycotic aneurysm in renal transplant recipients.
Journal of vascular surgery. 53(3):802-4 [DOI] 10.1016/j.jvs.2010.10.102. [PMID] 21215589.
2010
Adenosine A2A receptor activation on CD4+ T lymphocytes and neutrophils attenuates lung ischemia-reperfusion injury.
The Journal of thoracic and cardiovascular surgery. 139(2):474-82 [DOI] 10.1016/j.jtcvs.2009.08.033. [PMID] 19909990.
2010
Tissue-derived proinflammatory effect of adenosine A2B receptor in lung ischemia-reperfusion injury.
The Journal of thoracic and cardiovascular surgery. 140(4):871-7 [DOI] 10.1016/j.jtcvs.2010.06.051. [PMID] 20659747.
2009
CD4+ T lymphocytes mediate acute pulmonary ischemia-reperfusion injury.
The Journal of thoracic and cardiovascular surgery. 137(3):695-702; discussion 702 [DOI] 10.1016/j.jtcvs.2008.10.044. [PMID] 19258091.
2009
Enhanced fibrinolysis protects against lung ischemia-reperfusion injury.
The Journal of thoracic and cardiovascular surgery. 137(5):1241-8 [DOI] 10.1016/j.jtcvs.2008.12.029. [PMID] 19379998.
2009
NADPH oxidase in bone marrow-derived cells mediates pulmonary ischemia-reperfusion injury.
American journal of respiratory cell and molecular biology. 40(3):375-81 [DOI] 10.1165/rcmb.2008-0300OC. [PMID] 18787174.
2009
Protection from pulmonary ischemia-reperfusion injury by adenosine A2A receptor activation.
Respiratory research. 10 [DOI] 10.1186/1465-9921-10-58. [PMID] 19558673.
2008
Inflammatory lung injury after cardiopulmonary bypass is attenuated by adenosine A(2A) receptor activation.
The Journal of thoracic and cardiovascular surgery. 136(5):1280-7; discussion 1287 [DOI] 10.1016/j.jtcvs.2008.07.010. [PMID] 19026816.
2007
Proinflammatory response of alveolar epithelial cells is enhanced by alveolar macrophage-produced TNF-alpha during pulmonary ischemia-reperfusion injury.
American journal of physiology. Lung cellular and molecular physiology. 293(1):L105-13 [PMID] 17416740.
View on: PubMed
2007
Sustained elevation of intracellular cGMP causes oxidative stress triggering calpain-mediated apoptosis in photoreceptor degeneration.
Current eye research. 32(3):259-69 [PMID] 17453946.
View on: PubMed
2006
Alveolar macrophage activation is a key initiation signal for acute lung ischemia-reperfusion injury.
American journal of physiology. Lung cellular and molecular physiology. 291(5):L1018-26 [PMID] 16861385.
View on: PubMed
2006
Multiple, parallel cellular suicide mechanisms participate in photoreceptor cell death.
Experimental eye research. 83(2):380-9 [PMID] 16626700.
View on: PubMed

Grants

Jan 2021 ACTIVE
Lung IDO-1+ TNFR2+ cDC2 subset in control of lung mucosal tolerance: Mechanism and Application
Role: Co-Investigator
Funding: NATL INST OF HLTH NHLBI
Oct 2020 ACTIVE
Novel biomarkers for alpha 1-antitrypsin mediated liver disease in circulating exosomes
Role: Other
Funding: ALPHA ONE FOU
Jul 2020 ACTIVE
Novel specialized pro-resolving lipid mediators in resolution of aortic aneurysms and rupture
Role: Principal Investigator
Funding: NATL INST OF HLTH NHLBI
Jun 2020 ACTIVE
Role of myeloid-derived suppressor cells in aortic aneurysms and rupture
Role: Principal Investigator
Funding: FL DEPT OF HLTH BIOMED RES PGM/J&E KING
Jul 2019 ACTIVE
Raab Fund – Research Project
Role: Principal Investigator
Funding: UF FOU
Aug 2018 – Jul 2020
Role of Neutrophil Extracellular Traps in AAA Pathogenesis
Role: Co-Investigator
Funding: NATL INST OF HLTH NHLBI
Jul 2018 – Jun 2020
Gender Differences in Experimental Aortic Aneurysms
Role: Co-Investigator
Funding: NATL INST OF HLTH NHLBI
Jul 2018 ACTIVE
Pannexin-1 signaling in abdominal aortic aneurysms
Role: Principal Investigator
Funding: NATL INST OF HLTH NHLBI
Jun 2018 – Dec 2018
Protective role of stem cell-derived microvesicles and microRNAs in aortic aneurysms
Role: Principal Investigator
Funding: AMER HEART ASSOCIATION

Education

PhD
2012 · University of Virginia
MBBS
1998 · MR Medical College, Gulbarga, Karnataka, India

Teaching Profile

Courses Taught
2020-2021
DEN5120C Physiology
2020
GMS6683 Fundamentals of Vascular Physiology and Pathology

Contact Details

Phones:
Business:
(352) 294-8660
Emails: